TY - JOUR AU - Trussart, M AU - Yus, E AU - Martinez, S AU - Bau, D AU - Tahara, YO AU - Pengo, T AU - Widjaja, M AU - Kretschmer, S AU - Swoger, J AU - Djordjevic, S AU - Turnbull, L AU - Whitchurch, C AU - Miyata, M AU - Marti-Renom, MA AU - Lluch-Senar, M AU - Serrano, L Y2 - 2020/12/22 Y1 - 2017/03/08 SN - 2041-1723 UR - http://hdl.handle.net/11343/258300 AB - DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems. LA - English PB - NATURE PUBLISHING GROUP T1 - Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae DO - 10.1038/ncomms14665 IS - Nature Communications VL - 8 IS - 1 L1 - /bitstream/handle/11343/258300/PMC5344976.pdf?sequence=1&isAllowed=y ER -