TY  - JOUR
AU  - Trussart, M
AU  - Yus, E
AU  - Martinez, S
AU  - Bau, D
AU  - Tahara, YO
AU  - Pengo, T
AU  - Widjaja, M
AU  - Kretschmer, S
AU  - Swoger, J
AU  - Djordjevic, S
AU  - Turnbull, L
AU  - Whitchurch, C
AU  - Miyata, M
AU  - Marti-Renom, MA
AU  - Lluch-Senar, M
AU  - Serrano, L
Y2  - 2020/12/22
Y1  - 2017/03/08
SN  - 2041-1723
UR  - http://hdl.handle.net/11343/258300
AB  - DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
LA  - English
PB  - NATURE PUBLISHING GROUP
T1  - Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
DO  - 10.1038/ncomms14665
IS  - Nature Communications
VL  - 8
IS  - 1
L1  - /bitstream/handle/11343/258300/PMC5344976.pdf?sequence=1&isAllowed=y
ER  -