TY  - JOUR
AU  - Wijaya, JC
AU  - Khanabdali, R
AU  - Georgiou, HM
AU  - Kokkinos, M
AU  - James, PF
AU  - Brennecke, SP
AU  - Kalionis, B
Y2  - 2021/02/03
Y1  - 2020/08/01
SN  - 1360-9947
UR  - http://hdl.handle.net/11343/258928
AB  - Ageing and parturition share common pathways, but their relationship remains poorly understood. Decidual cells undergo ageing as parturition approaches term, and these age-related changes may trigger labour. Mesenchymal stem/stromal cells (MSCs) are the predominant stem cell type in the decidua. Stem cell exhaustion is a hallmark of ageing, and thus ageing of decidual MSCs (DMSCs) may contribute to the functional changes in decidual tissue required for term spontaneous labour. Here, we determine whether DMSCs from patients undergoing spontaneous onset of labour (SOL-DMSCs) show evidence of ageing-related functional changes compared with those from patients not in labour (NIL-DMSCs), undergoing Caesarean section. Placentae were collected from term (37-40 weeks of gestation), SOL (n = 18) and NIL (n = 17) healthy patients. DMSCs were isolated from the decidua basalis that remained attached to the placenta after delivery. DMSCs displayed stem cell-like properties and were of maternal origin. Important cell properties and lipid profiles were assessed and compared between SOL- and NIL-DMSCs. SOL-DMSCs showed reduced proliferation and increased lipid peroxidation, migration, necrosis, mitochondrial apoptosis, IL-6 production and p38 MAPK levels compared with NIL-DMSCs (P < 0.05). SOL- and NIL-DMSCs also showed significant differences in lipid profiles in various phospholipids (phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine), sphingolipids (ceramide, sphingomyelin), triglycerides and acyl carnitine (P < 0.05). Overall, SOL-DMSCs had altered lipid profiles compared with NIL-DMSCs. In conclusion, SOL-DMSCs showed evidence of ageing-related reduced functionality, accumulation of cellular damage and changes in lipid profiles compared with NIL-DMSCs. These changes may be associated with term spontaneous labour.
LA  - English
PB  - Oxford University Press (OUP)
T1  - Functional changes in decidual mesenchymal stem/stromal cells are associated with spontaneous onset of labour
DO  - 10.1093/molehr/gaaa045
IS  - Molecular Human Reproduction
VL  - 26
IS  - 8
SP  - 636-651
L1  - /bitstream/handle/11343/258928/Wijaya%20Mol%20Hum%20Reprod%202020.pdf?sequence=2&isAllowed=n
ER  -