TY  - JOUR
AU  - Schlissel, MS
AU  - Corcoran, LM
AU  - Baltimore, D
Y2  - 2021/02/04
Y1  - 1991/03/01
SN  - 0022-1007
UR  - http://hdl.handle.net/11343/259117
AB  - Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.
LA  - eng
PB  - Rockefeller University Press
T1  - Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.
DO  - 10.1084/jem.173.3.711
IS  - Journal of Experimental Medicine
VL  - 173
IS  - 3
SP  - 711-720
L1  - /bitstream/handle/11343/259117/PMC2118835.pdf?sequence=1&isAllowed=y
ER  -