TY  - JOUR
AU  - Duncan, LM
AU  - Timms, RT
AU  - Zavodszky, E
AU  - Cano, F
AU  - Dougan, G
AU  - Randow, F
AU  - Lehner, PJ
Y2  - 2021/02/05
Y1  - 2012/06/22
SN  - 1932-6203
UR  - http://hdl.handle.net/11343/260026
AB  - The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation. Non-lethal haploid genetic screens are widely applicable to identify genes involved in essentially any cellular pathway.
LA  - English
PB  - PUBLIC LIBRARY SCIENCE
T1  - Fluorescence-Based Phenotypic Selection Allows Forward Genetic Screens in Haploid Human Cells
DO  - 10.1371/journal.pone.0039651
IS  - PLoS One
VL  - 7
IS  - 6
L1  - /bitstream/handle/11343/260026/PMC3382162.pdf?sequence=1&isAllowed=y
ER  -