TY  - JOUR
AU  - Cerruti, B
AU  - Puliafito, A
AU  - Shewan, AM
AU  - Yu, W
AU  - Combes, AN
AU  - Little, MH
AU  - Chianale, F
AU  - Primo, L
AU  - Serini, G
AU  - Mostov, KE
AU  - Celani, A
AU  - Gamba, A
Y2  - 2021/02/05
Y1  - 2013/10/28
SN  - 0021-9525
UR  - http://hdl.handle.net/11343/260099
AB  - The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell-cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell-cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis.
LA  - English
PB  - ROCKEFELLER UNIV PRESS
T1  - Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures
DO  - 10.1083/jcb.201305044
IS  - The Journal of Cell Biology
VL  - 203
IS  - 2
SP  - 359-372
L1  - /bitstream/handle/11343/260099/PMC3812962.pdf?sequence=1&isAllowed=y
ER  -