TY  - JOUR
AU  - Tree, JJ
AU  - Granneman, S
AU  - McAteer, SP
AU  - Tollervey, D
AU  - Gally, DL
Y2  - 2021/02/05
Y1  - 2014/07/17
SN  - 1097-2765
UR  - http://hdl.handle.net/11343/260447
AB  - In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.
LA  - eng
PB  - Elsevier BV
T1  - Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.
DO  - 10.1016/j.molcel.2014.05.006
IS  - Molecular Cell
VL  - 55
IS  - 2
SP  - 199-213
L1  - /bitstream/handle/11343/260447/PMC4104026.pdf?sequence=1&isAllowed=y
ER  -