TY  - JOUR
AU  - Ngo, T
AU  - Duraisingh, M
AU  - Reed, M
AU  - Hipgrave, D
AU  - Biggs, B
AU  - Cowman, AF
Y2  - 2014/05/21
Y1  - 2003/03/01
SN  - 0002-9637
UR  - http://hdl.handle.net/11343/26163
AB  - We have analyzed artemisinin sensitivity in Plasmodium falciparum isolates obtained from patients in South Vietnam and show that artemisinin sensitivity does not differ before and after drug treatment. There was an increase in the level of mefloquine resistance in the isolates after drug treatment that was concomitant with a decrease in chloroquine resistance, suggesting that treatment with artemisinin has selected for increased mefloquine resistance. Mutations in the pfmdr1 gene, previously shown to be associated with sensitivity to mefloquine, were selected against. All isolates resistant to chloroquine encoded Thr-76 in the pfcrt gene consistent with an essential role in the mechanism of chloroquine resistance. Mutations in pfmdr1 also were linked to chloroquine resistance. High levels of mutation in dhfr and dhps genes, which have previously been associated with Fansidar resistance, also were found, suggesting that this drug would not be useful for malaria control in this part of Vietnam.
N1  - application/pdf
LA  - English
PB  - AMER SOC TROP MED & HYGIENE
KW  - Clinical Pharmacology and Therapeutics; Infectious Diseases; Infectious Diseases
T1  - Analysis of pfcrt, pfmdr1, dhfr, and DHPS mutations and drug sensitivities in Plasmodium falciparum isolates from patients in Vietnam before and after treatment with artemisinin
DO  - 10.4269/ajtmh.2003.68.350
IS  - AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
VL  - 68
IS  - 3
SP  - 350-356
L1  - /bitstream/handle/11343/26163/115926_4968.pdf?sequence=1&isAllowed=n
ER  -