TY  - JOUR
AU  - Kedzierska, K
AU  - La Gruta, NL
AU  - Davenport, MP
AU  - Turner, SJ
AU  - Doherty, PC
Y2  - 2014/05/21
Y1  - 2005/08/09
SN  - 0027-8424
UR  - http://hdl.handle.net/11343/26430
AB  - Prior analysis has characterized the clonal characteristics of effector CD8(+) T cells specific for the prominent influenza A virus nucleoprotein (NP) and acid polymerase (PA) peptides presented by H2D(b). Using a single-cell approach and determination of CDR3beta profiles, a limited, predominantly "public" repertoire was found for CD8(+)D(b)NP(366)(+)Vbeta8.3+ cells, whereas diverse and "private" T cell antigen receptor (TCR)beta clonotypes were typical of the CD8(+)D(b)PA(224)(+)Vbeta7+ response. This single-cell approach has now been used to relate the contributions of particular clonotypes (or affinities) to high-avidity TCRs, as defined by binding under conditions of limiting tetramer availability. At least by the measure of CDR3beta usage, no difference could be found between total and high-avidity populations in the spectrum of TCR-pMHC affinities throughout the limited, and relatively public, CD8(+)D(b)NP(366)(+)Vbeta8.3+ populations. Conversely, the more even (by clone size), diverse, and private CD8(+)D(b)PA(224)(+)Vbeta7+ response was characterized by the clear partitioning of the largest T cell clones in the high-avidity compartment. These results suggest that the relatively constrained CD8(+)D(b)NP(366)(+)Vbeta8.3+ set utilizes a relatively narrow range of affinities, whereas the broader CD8(+)D(b)PA(224)(+)Vbeta7+ response is induced at a range of TCR-pMHC affinities. Thus, whereas TCR sequence (or affinity) appears to contribute substantially to the avidity profile of diverse virus-specific CD8+ populations, other mechanisms may be prominent where the TCR spectrum is more limited.
N1  - application/pdf
LA  - English
PB  - NATL ACAD SCIENCES
KW  - Cellular Immunology; Immune System and Allergy
T1  - Contribution of T cell receptor affinity to overall avidity for virus-specific CD8(+) T cell responses
DO  - 10.1073/pnas.0504851102
IS  - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
VL  - 102
IS  - 32
SP  - 11432-11437
L1  - /bitstream/handle/11343/26430/116226_5275.pdf?sequence=1&isAllowed=n
ER  -