TY  - JOUR
AU  - Malkoski, M
AU  - Dashper, SG
AU  - O'Brien-Simpson, NM
AU  - Talbo, GH
AU  - Macris, M
AU  - Cross, KJ
AU  - Reynolds, EC
Y2  - 2014/05/21
Y1  - 2001/08/01
SN  - 0066-4804
UR  - http://hdl.handle.net/11343/26560
AB  - Caseinomacropeptide (CMP) is a heterogeneous C-terminal fragment (residues 106 to 169) of bovine milk kappa-casein composed of glycosylated and phosphorylated forms of different genetic variants. We have demonstrated that CMP has growth-inhibitory activity against the oral opportunistic pathogens Streptococcus mutans and Porphyromonas gingivalis and against Escherichia coli. CMP was fractionated using reversed-phase high-performance liquid chromatography (RP-HPLC), and each fraction was tested for activity against S. mutans in a 96-well-plate broth assay. Fractions were characterized by N-terminal sequence analysis and mass spectrometry. The active form of CMP was shown to be the nonglycosylated, phosphorylated kappa-casein (residues 106 to 169) [kappa-casein(106--169)], which we have designated kappacin. Endoproteinase Glu-C was used to hydrolyze CMP, and the generated peptides were separated using RP-HPLC and gel filtration-HPLC and then tested for activity against S. mutans. The peptide Ser(P)(149)kappa-casein-A(138--158) was the only peptide generated by endoproteinase Glu-C digestion that exhibited growth-inhibitory activity. Peptides corresponding to the sequences of the inhibitory peptide Ser(P)(149)kappa-casein-A(138--158) and its nonphosphorylated counterpart kappa-casein-A(138--158) were chemically synthesized and tested for antibacterial activity. The synthetic Ser(P)(149) kappa-casein-A(138--158) displayed growth-inhibitory activity against S. mutans (MIC, 59 microg/ml [26 microM]). The nonphosphorylated peptide, however, did not inhibit growth at the concentrations tested, indicating that phosphorylation is essential for activity.
N1  - application/pdf
LA  - English
PB  - AMER SOC MICROBIOLOGY
KW  - Bacteriology ; Infectious Agents; Dentistry not elsewhere classified ; Treatments (e.g. Chemicals
KW  - Antibiotics); Dental Health
T1  - Kappacin, a novel antibacterial peptide from bovine milk
DO  - 10.1128/AAC.45.8.2309-2315.2001
IS  - ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
VL  - 45
IS  - 8
SP  - 2309-2315
L1  - /bitstream/handle/11343/26560/116364_5410.pdf?sequence=1&isAllowed=n
ER  -