TY  - JOUR
AU  - Sutton, P
AU  - Danon, SJ
AU  - Walker, M
AU  - Thompson, LJ
AU  - Wilson, J
AU  - Kosaka, T
AU  - Lee, A
Y2  - 2014/05/21
Y1  - 2001/10/01
SN  - 0017-5749
UR  - http://hdl.handle.net/11343/26608
AB  - BACKGROUND AND AIMS: Helicobacter pylori is a major cause of peptic ulcers and gastric cancer. Vaccine development is progressing but there is concern that immunisation may exacerbate Helicobacter induced gastritis: prophylactic immunisation followed by challenge with H felis or H pylori can induce a more severe gastritis in mice than seen with infection alone. The aim of this study was to investigate the relationship between immunity to Helicobacter infection and post-immunisation gastritis. METHODS: (1) C57BL/6 mice were prophylactically immunised before challenge with either H felis or H pylori. Histopathology and colonisation were assessed one month post-challenge. (2) C57BL/6 mice were prophylactically immunised against H felis infection and gastritis assessed up to 18 months post-challenge. RESULTS: Prophylactic immunisation induced a reduction in bacterial colonisation following H felis challenge which was associated with increased severity of active gastritis with neutrophil infiltration and atrophy. However, immunised mice challenged with H pylori SS1 had little evidence of pathology. Long term follow up showed that post-immunisation gastritis was evident at three months. However, from six months onwards, although immunised/challenged mice still developed gastritis, there was no significant difference between inflammation in these mice and infected controls. Post-immunisation gastritis was not associated with the serum antibody response. Immunisation prevented the formation of secondary lymphoid aggregates in the gastric tissue. CONCLUSION: The H felis mouse model of post-immunisation gastritis is the most extreme example of this type of pathology. We have shown in this model that post-immunisation gastritis is a transient event which does not produce long term exacerbation of pathology.
N1  - application/pdf
LA  - English
PB  - BRITISH MED JOURNAL PUBL GROUP
KW  - Immunology not elsewhere classified; Prevention - Biologicals (e.g. Vaccines)
T1  - Post-immunisation gastritis and Helicobacter infection in the mouse: a long term study
DO  - 10.1136/gut.49.4.467
IS  - GUT
VL  - 49
IS  - 4
SP  - 467-473
L1  - /bitstream/handle/11343/26608/116819_5461.pdf?sequence=1&isAllowed=n
ER  -