TY  - JOUR
AU  - Crowe, SR
AU  - Turner, SJ
AU  - Miller, SC
AU  - Roberts, AD
AU  - Rappolo, RA
AU  - Doherty, PC
AU  - Ely, KH
AU  - Woodland, DL
Y2  - 2014/05/21
Y1  - 2003/08/04
SN  - 0022-1007
UR  - http://hdl.handle.net/11343/26616
AB  - The specificity of CD8+ T cell responses can vary dramatically between primary and secondary infections. For example, NP366-374/Db- and PA224-233/Db-specific CD8+ T cells respond in approximately equal numbers to a primary influenza virus infection in C57BL/6 mice, whereas NP366-374/Db-specific CD8+ T cells dominate the secondary response. To investigate the mechanisms underlying this changing pattern of immunodominance, we analyzed the role of antigen presentation in regulating the specificity of the T cell response. The data show that both dendritic and nondendritic cells are able to present the NP366-374/Db epitope, whereas only dendritic cells effectively present the PA224-233/Db epitope after influenza virus infection, both in vitro and in vivo. This difference in epitope expression favored the activation and expansion of NP366-374/Db-specific CD8+ memory T cells during secondary infection. The data also show that the immune response to influenza virus infection may involve T cells specific for epitopes, such as PA224-233/Db, that are poorly expressed at the site of infection. In this regard, vaccination with the PA224-233 peptide actually had a detrimental effect on the clearance of a subsequent influenza virus infection. Thus, differential antigen presentation impacts both the specificity of the T cell response and the efficacy of peptide-based vaccination strategies.
N1  - application/pdf
LA  - English
PB  - ROCKEFELLER UNIV PRESS
KW  - Cellular Immunology; Immune System and Allergy
T1  - Differential antigen presentation regulates the changing patterns of CD8(+) T cell immunodominance in primary and secondary influenza virus infections
DO  - 10.1084/jem.20022151
IS  - Journal of Experimental Medicine
VL  - 198
IS  - 3
SP  - 399-410
ER  -