TY  - JOUR
AU  - Winnall, WR
AU  - Ali, U
AU  - O'Bryan, MK
AU  - Hirst, JJ
AU  - Whiley, PAF
AU  - Muir, JA
AU  - Hedger, MP
Y2  - 2014/05/21
Y1  - 2007/05/01
SN  - 0006-3363
UR  - http://hdl.handle.net/11343/28982
AB  - Prostaglandins (PGs), particularly PGE(2), have been implicated in the control of testicular steroidogenesis, spermatogenesis, and local immunity. However, virtually nothing is known about the expression or activity of the prostaglandin-endoperoxide synthases (PTGSs; also referred to as the cyclooxygenases), the specific rate-limiting enzymes responsible for PG production, in the adult testis. This activity was investigated in rats under normal conditions and during lipopolysaccharide-induced inflammation using quantitative real-time PCR, in situ hybridization, Western blotting, and PGE(2) measurements by ELISA. The mRNA for both the "constitutive" Ptgs1 and the "inducible" Ptgs2 forms was detected in multiple testicular cell types. Testicular Ptgs2 expression was substantially higher than that of Ptgs1, and testicular production of PGE(2) in vitro was found to be suppressed by a specific PTGS2 inhibitor (NS-398), but not by an inhibitor of PTGS1. Further investigation indicated that 1) PGE(2) production in the adult testis is attributable to constitutive expression of PTGS2 by somatic (Leydig cells and Sertoli cells) and spermatogenic cells; 2) testicular macrophages constitutively produce relatively low levels of PTGS2 and PGE(2) but are the only cell type to respond significantly to an inflammatory stimulus by increasing production of PGE(2); and 3) testicular PTGS2 expression and intratesticular PGE(2) levels are only marginally affected by acute inflammation. These data point toward a previously unanticipated maintenance role for the "inducible" PTGS2 enzyme in normal testicular function, as well as an anomalous response of testicular PTGS2 to inflammatory stimuli. Both observations are consistent with the reduced capacity of the testis to initiate and support inflammatory reactions.
N1  - application/pdf
LA  - English
PB  - OXFORD UNIV PRESS INC
KW  - Cellular Immunology; Reproduction; Immune System and Allergy; Reproductive System and Disorders
T1  - Constitutive expression of prostaglandin-endoperoxide synthase 2 by somatic and spermatogenic cells is responsible for prostaglandin E-2 production in the adult rat testis
DO  - 10.1095/biolreprod.106.053124
IS  - Biology of Reproduction
VL  - 76
IS  - 5
SP  - 759-768
L1  - /bitstream/handle/11343/28982/271254_9945.pdf?sequence=1&isAllowed=n
ER  -