TY  - JOUR
AU  - Zuccala, ES
AU  - Gout, AM
AU  - Dekiwadia, C
AU  - Marapana, DS
AU  - Angrisano, F
AU  - Turnbull, L
AU  - Riglar, DT
AU  - Rogers, KL
AU  - Whitchurch, CB
AU  - Ralph, SA
AU  - Speed, TP
AU  - Baum, J
Y2  - 2014/05/22
Y2  - 2012/08/27
Y2  - 2012/08/27
Y2  - 2012/08/27
Y1  - 2012/09/25
SN  - 1932-6203
UR  - http://hdl.handle.net/11343/32738
AB  - Host cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pathogens. For most species, including the etiological agents of malaria and toxoplasmosis, infection requires active host-cell invasion dependent on formation of a tight junction - the organising interface between parasite and host cell during entry. Formation of this structure is not, however, shared across all Apicomplexa or indeed all parasite lifecycle stages. Here, using an in silico integrative genomic search and endogenous gene-tagging strategy, we sought to characterise proteins that function specifically during junction-dependent invasion, a class of proteins we term invasins to distinguish them from adhesins that function in species specific host-cell recognition. High-definition imaging of tagged Plasmodium falciparum invasins localised proteins to multiple cellular compartments of the blood stage merozoite. This includes several that localise to distinct subcompartments within the rhoptries. While originating from the same organelle, however, each has very different dynamics during invasion. Apical Sushi Protein and Rhoptry Neck protein 2 release early, following the junction, whilst a novel rhoptry protein PFF0645c releases only after invasion is complete. This supports the idea that organisation of proteins within a secretory organelle determines the order and destination of protein secretion and provides a localisation-based classification strategy for predicting invasin function during apicomplexan parasite invasion.
LA  - English
PB  - PUBLIC LIBRARY SCIENCE
KW  - cell infection
KW  - intracellular pathogens
KW  - malaria parasite
KW  - proteins
T1  - Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite
DO  - 10.1371/journal.pone.0046160
IS  - PLOS ONE
VL  - 7
IS  - 9
L1  - /bitstream/handle/11343/32738/288857_Subcompartmentalisation%20of%20proteins_PLOS%20one_CC.pdf?sequence=1&isAllowed=y
ER  -