TY  - JOUR
AU  - Onimaru, H
AU  - Dutschmann, M
Y2  - 2014/05/22
Y2  - 2011/10/18
Y1  - 2012/01/01
SN  - 1880-6546
UR  - http://hdl.handle.net/11343/33194
AB  - The parafacial respiratory group (pFRG) is thought to be involved in respiratory rhythm generation in neonates. This subgroup expresses the transcription factor, Phox2b, and contains intrinsically CO(2) sensitive neurons. Calcium imaging has been widely used for analysis of neuronal activity at the cellular and network level. In the present study, we applied calcium imaging to analyze neuronal activity of the most-rostral pFRG in an in vitro brainstem-spinal cord preparation from neonatal rats. We detected strong pre-inspiratory neuron activity in the most rostral pFRG, suggesting that significant numbers of pre-inspiratory neurons are localized in the ventrolateral medulla near the rostral end of the medulla. We show that usage of calcium imaging would be very useful for analysis of neuronal activity over different time scales, and discuss the advantages and disadvantages of this method.
N1  - application/pdf
LA  - English
PB  - SPRINGER JAPAN KK
KW  - Neurosciences not elsewhere classified; Clinical Health (Organs
KW  - Diseases and Abnormal Conditions) not elsewhere classified
T1  - Calcium imaging of neuronal activity in the most rostral parafacial respiratory group of the newborn rat
DO  - 10.1007/s12576-011-0179-2
IS  - Journal of Physiological Sciences
VL  - 62
IS  - 1
SP  - 71-77
L1  - /bitstream/handle/11343/33194/298269_17324.pdf?sequence=1&isAllowed=n
ER  -