Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival
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Fox, D
Date
2009-03-30
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PLoS ONE
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PUBLIC LIBRARY SCIENCE
Melbourne Authors
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Medical Biology (W.E.H.I.)
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Journal Article
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Creagh, E. M., Brumatti, G., Sheridan, C., Duriez, P. J., Taylor, R. C., Cullen, S. P., Adrain, C. & Martin, S. J. (2009). Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival. Plos One, 4 (3), https://doi.org/10.1371/journal.pone.0005055.
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Abstract
Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.