Early appearance of germinal center-derived memory B cells and plasma cells in blood after primary immunization
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2005-02-21
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Journal of Experimental Medicine (JEM)
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ROCKEFELLER UNIV PRESS
Melbourne Authors
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Microbiology and Immunology
Medical Biology (W.E.H.I.)
Medical Biology (W.E.H.I.)
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Journal Article
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Blink, E. J., Light, A., Kallies, A., Nutt, S. L., Hodgkin, P. D. & Tarlinton, D. M. (2005). Early appearance of germinal center-derived memory B cells and plasma cells in blood after primary immunization. Journal of Experimental Medicine, 201 (4), pp.545-554. https://doi.org/10.1084/jem.20042060.
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Abstract
Immunization with a T cell-dependent antigen elicits production of specific memory B cells and antibody-secreting cells (ASCs). The kinetic and developmental relationships between these populations and the phenotypic forms they and their precursors may take remain unclear. Therefore, we examined the early stages of a primary immune response, focusing on the appearance of antigen-specific B cells in blood. Within 1 wk, antigen-specific B cells appear in the blood with either a memory phenotype or as immunoglobulin (Ig)G1 ASCs expressing blimp-1. The memory cells have mutated V(H) genes; respond to the chemokine CXCL13 but not CXCL12, suggesting recirculation to secondary lymphoid organs; uniformly express B220; show limited differentiation potential unless stimulated by antigen; and develop independently of blimp-1 expression. The antigen-specific IgG1 ASCs in blood show affinity maturation paralleling that of bone marrow ASCs, raising the possibility that this compartment is established directly by blood-borne ASCs. We find no evidence for a blimp-1-expressing preplasma memory compartment, suggesting germinal center output is restricted to ASCs and B220(+) memory B cells, and this is sufficient to account for the process of affinity maturation.